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Protein Instability Index
Calculator |
Dipeptide composition in a protein's
primary structure is predicted to play a role in protein stability.
PEST hypothesis and Protein Instability Index (PII) are two proposals
relating the protein stabilty to the primary strucrure (1, 2). The
mechanism of degradation in relation to the primary sequence is not
understood well. PII is a statistical analytical procedure based on
Dipeptide Instability Weight Value (DIWV)(2).
While the above mentioned hypotheses
considers only the in vivo protein stabilty, one of
the proteins we cloned overespressed in our lab was
caluculated to have a PII of 40.10, a value just outside the stable
region (PII of 1 - 40 are considered relatively stable). As predicted
our expressed protein degraded rapidly (in days) upon storage at 4
ºC.
We wrote a computer program based on
Guruprasad et al.'s work, in order to help ourselves in
calculating PII for our experiments. We were able to calculate the
exact predited PII value for our protein.
We are making the program available here
for anyone to use. We are not responsible for any errors in the
program. We appreciate if you could share your results and inform us if
you find any problem with the calculator to improve it.
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References: |
Guruprasad, K., B. V. Reddy, et al. (1990). "Correlation between stability of a protein and its dipeptide composition: a novel approach for predicting in vivo stability of a protein from its primary sequence." Protein Eng 4(2): 155-61. |