BIO 5406
McGilliard

Reading Assignment:

     Hadley, pp. 67-90.

     Witherspoon, L.  Immunoassay: The evolution of a revolution in disease diagnosis.  Modern
          Medicine 57(3): 68-72, 1989.
 

     Francois, E., Wang, D.Y., Fulthorpe, R., Liss, S.N., and Edwards, E.A.  DNA microarrays for

          detecting endocrine-disrupting compounds.  Biotechnology Advances 22: 17-26, 2003.

          http://snhs-plin.barry.edu/Research/Francois_endocrine_disruptor_DNA_microarray_2003.pdf

Objectives:

     After reviewing your lecture notes and reading assignment, you should be able to:

     1.  Describe and give an example of the classic technique of endocrinology.

          Lab -- 1/21

          Text -- pp. 67, 69-70

     2.  Describe the principles and uses of each of the following experimental techniques,

          with examples from endocrinology:
          a.  Bioassay.
          b.  Radioimmunoassay (RIA).
          c.  Enzyme-linked colorimetric immunoassay.
          d.  Immunocytochemistry.
          e.  DNA microarrays.

          Lab/Lecture -- 1/21, 1/22
          Text -- pp. 71-77

          Figures -- 4.2, 4.3, 4.4, 8.18
          Witherspoon paper

          Francois paper

          DNA Microarray Animation

     3.  Give an example of the use of recombinant DNA technology in the advancement of

          endocrinology.

          Lab -- 1/21

          Text -- pp. 85-86

          Figure -- 4.11

     4.  Discuss the use of transgenic and knock-out animals in endocrinology research.

          Lab -- 1/21

          Text -- pp. 86-88

          Figure -- 4.12

     5.  Throughout the semester, be prepared to design experiments which demonstrate
          various properties of endocrine glands or principles of endocrinology.

     6.  Describe the contributions of Rosalyn Yalow and Solomon Berson to the field of
          endocrinology.
 

          Lab -- 1/21
          Text -- pg. 6
          1977 Nobel Press Release