Bio 3950      Autumn 2007 – Mullin

Vertebrate Natural History

Introduction

A. How does science work? Hypothetico-deductive reasoning:

1. Observation

2. Hypothesis -- this proposes a possible cause (or several causes)

3. Test hypothesis

4. Retain/Refute/Revise hypothesis

(5. Retest revised hypothesis)

All hypotheses: (a) should be testable; and, (b) can be eliminated via testing.

Biological phenomena are based on a hierarchical organization of living things, the structures within them, and the roles they play in the environment -- subatomic particles & atoms > molecules > organelles > cells > tissues > organs > systems > multicellular organisms > populations > communities > ecosystems > biomes > biosphere

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Distinction & Classification

Taxonomic hierarchy:

Kingdom, Phylum, Class, Order, Family, Genus, species

The hierarchy is based on a system called binomial nomenclature. This system assigns each organismal species with a two-part Latin name -- a Genus name, and a specific epithet. However, no two species have the same binomial epithet.

Why is there so much diversity? Why are there two different types of bacteria/mushrooms/snakes/etc. in a particular place on Earth, when there just as easily could be one?? Historical changes in environmental conditions have caused changes in ancestral forms of organisms which produced more than one type of descendant.

Evolution by process of natural selection --

: the environment changes

: organisms produce far more offspring than are capable of surviving

: some of those offspring possess traits which are better suited for the current environmental conditions than others.

: those offspring better adapted to current environmental conditions will out-compete those which are not for available resources, and hence survive to reproduce (this passes their genes on to future generations).

An important concept to grasp about evolutionary processes is TIME, and an appreciation for how much of it must elapse in order to produce evolutionary change.

One global concern is human activities accelerating the rate of environmental change to a level where other organisms cannot keep pace and thus go extinct.

How do you tell the difference between taxonomic groups?

a) morphology: similar measurements on variety of char's = relatedness

b) behavior: similar postures/displays/strategies = close relatedness

c) ecology: similar habitat req's, interspp. assoc. = close relatedness

d) biochemical: genetic similarity = relatedness

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Evolutionary History & Biogeography

Global conditions past & present –

4.8 bya = formation of Earth

3.5 bya = oldest recognizable biotic forms; atmosphere 02-deficient.

2.6 bya = 1st algæ, beginning of 02 build-up in atmosphere

1.6 bya = 1st eukaryotes; Pangæa formation begins

680 mya = 1st multicellular organisms, rapid radiation during Cambrian

525 mya = 1st vertebrate ancestor

500 mya = 1st fish ancestor

440 mya = 1st land plants

415 mya = 1st land invertebrates

360 mya = 1st amphibians, trees; beginning of coal deposit formation

320 mya = 1st reptiles; Pangæa formation complete; Permian-Triassic extinction (of 83% of known Genera)

210 mya = Triassic-Jurassic extinction (of 48 % of known Genera); 1st marsupials, birds

140 mya = 1st angiosperms; Pangæa breaks into Laurasia (N hemisphere) and Gondwonaland (S hemisphere)

65 mya = Alp and Rocky Mtn orogeny; 1st placentals; Cretaceous-Tertiary extinction (of 50 % of known Genera)

4.8 mya = 1st hominids; polar ice cap formation

1.2 mya = Homo erectus, tool use;1st of repeated glaciations

10 - 0.5 kya = anthropogenic extinction of Australian (85 %), North American (60 %), Madagascan/New Zealand (99 %) fauna.

 

The climate (temp., precip., photoperiod, wind) on any particular region affects the organisms found there. Local climates can be affected by (a) latitude, (b) proximity to ocean, and (c) altitudinal barriers. Global climates have changed during Earth's history (& effected taxa distribution) b/c:

a) plate tectonics –continents are "floating" on viscous mantle pool. @ time 1st vertebrates, 1 of 2 major continents of Pre-Cambrian (Laurasia) 6 broken up into 5 smaller continents; this facilitated taxa radiation.

b) glaciation – changes in polar ice area affect sea level, and therefore, climate & exposed land area.

 

Biogeographic Rules affecting extant endothermic taxa.

1. Bergman's Rule: greater indiv. size @ higher latitude, b/c lower temp. means indiv's should have lower SA:V to conserve heat.

2. Allen's Rule: shorter extremities in areas w/ greater temp. fluctuation b/c indiv's should have lower SA:V to conserve heat.

3. Gloger's Rule: indiv. w/ darker color in warm/humid areas, b/c there is more shading in tropics

4. Fahrenholz's Rule: symbiotic spp. (e.g., parasite-host) tend to evolve together, so similar spp. should have similar symbionts.

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If these rules can be used to explain size variation between popln's of indiv's w/in spp., then what explains sexual size dimorphism?

1. F needs to eat more prey to produce larger gametes

2. F needs to be larger to produce larger clutch size

3. F needs to be larger to avoid injury during ritualized courtship display

4. F needs to be larger to incubate clutch (oviparous taxa only)

5. M needs to be larger to defend resources

6. dichotomy exists to reduce competition for other resources.

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Phylum Chordata – four common features

(a) notochord - attachment site for muscles

(b) nerve cord - nervous system, may have anterior development (brain)

(c) pharyngeal gill slits - may form respiratory structures or be vestigial

(d) post-anal tail - may be vestigial

Subphylum Urochordata – filter-feeding tunicates w/ "tadpole" larvæ; paedomorphic larval form gave rise to other chordates.

Subphylum Cephalochordata – lancelets w/ segmented myomeres that aid in movement; gill slits for feeding (not respirat'n) & open circulatory system.

Subphylum Vertebrata – shared features

(a) vertebrae – serially arranged along dorsum = cartilage, bone (or absent)

(b) cranium – a skull surrounding brain = fibrous, cartilage, or bone

(c) neural crest – embryonic tissue that forms novel structures (esp. in head)

(d) enlarged, tripartite brain

These structures manifested increased size and activity levels in vertebrates which in turn promoted other adaptations: pharyngeal gills (fewer in #, but more complex) for respirat'n, true heart, muscularized g.i. tract, & discrete visceral organs (e.g., kidney, liver, pancreas).

Based on fossil evidence & physiology of extant forms, the 1st vertebrates arose in marine environments. Among early taxa, ostracoderms were 1st to have bone (head plates) and glomerular kidney for Ca+2 and P+2 uptake. Extant analogy = Superfamily Myxinoidea (extant hagfish) w/ equal ion concentration as seawater but w/out bone seen as proto-vertebrate condition.

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The Unity of Structure

Embryological system development

1. zygote — fertilized egg

2. blastula — formation of primitive coelom (hollow ball of many cells)

3. gastrula — formation of three tissue layers (3-layered ball)

a) ectoderm forms superficial layers, g.i. tract, nervous/sensory organs

b) endoderm forms g.i. tract & assoc. glands (e.g., liver, pancreas).

c) mesoderm forms muscle, skeletal, circulatory, urogenital organs

4. neurula — formation of notochord & dorsal nerve chord from ectoderm.

 

The germ layers lead to different tissue types:

1. Epithelial -- (a) membranous, func. for coverage

(b) glands: i. Endocrine glands secrete hormones into extracellular space

ii. Exocrine glands secrete material directly to target organ or outside body.

2. Connective -- tendons/ligaments func. for support

3. Vascular — circulate blood, gases, wastes

4. Muscle -- ability to contract for movement function

5. Nervous -- ability to conduct e- signals for sensory function.

 

Structural/protective elements......

Components of skeletal tissue:

1. cartilage -- embryonic bone; living cells that provide flexible support and connections between bones.

2. compact bone -- dense, strong bone serving as attachment site for muscles.

3. spongy bone -- porous, light-weight, highly-vascularized; bone marrow is located in the cavities and this is where blood cells are formed.

Divisions of the skeletal system:

1. appendicular -- the bones of the appendages (girdles & limbs)

2. axial -- the bones of the central axis (skull, spinal column, & ribs).

 

Functions of vertebrate skeleton:

1. support/protection of visceral organs in body as well as attachment site for muscle tissue.

2. production of cells in blood, including erythrocytes, leukocytes, platelets.

3. storage site for Ca and P

4. sensory perception -- the bones of the middle ear that transmit sound.

 

Specialized bone type — teeth are a highly-mineralized combo. of bone, enamel, and dentine. Types of root systems:

1. acrodont — fused to top of jaw margin

2. pleurodont — set on shelf on medial side of jaw

3. thecodont — set in sockets

 

Body movement is accomplished by muscles attaching to bones w/ bands of connective tissue called tendons. Skeletal muscles are arranged in antagonistic pairs such that the contraction of one muscle to flex a joint can be reversed by the contraction of another muscle to extend the same joint.

 

Types of muscle tissue:

1. skeletal muscle -- multinucleate, striated, voluntary contractions

2. smooth muscle -- mononucleate, non-striated, involuntary contraction, moves stuff through hollow organs.

3. cardiac muscle -- multinucleate, semi-striated, involuntary control

Muscle fiber (cell) structure consists of several subunits called myofibrils each of which has hundreds of bands of protein chains. One protein type, myosin, attaches to another protein type, actin, and pulls the two actin chains together, thus shortening the muscle.

The muscle fibers are stimulated by electrical signals from motor neurons.

 

Vertebrate muscular systems divided into 2 broad regions:

1. axial — folded blocks originating from the spinal column, 1° support

(a) epaxial — those articulating dorsal to the spinal column

(b) hypaxial — those articulating ventral to the spinal column

2. appendicular — originating from limb bones, 1° locomotion

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The Unity of Function

Acquisition of nutrients is a multi-step process:

1. ingestion -- food is brought into the mouth

2. digestion -- chemical breakdown via enzymes, acids and mastication (mechanical breakdown of food, typically w/ teeth)

4. absorption -- transport of small molecules (including water) from digestive cavity into cells or circulatory system.

5. elimination -- indigestible materials are expelled from the body.

 

Pathway of food: mouth (mastication, digestion of starch), to esophagus, to stomach (digestion of protein), to small intestine (digestion of fats & sugars, absorption), to large intestine (absorption), rectum, anus.

 

Accessory digestive organs:

1. salivary glands -- in mouth, secrete enzyme to digest starch.

2. Gall bladder -- w/in liver, produces bile salts to digest fats.

3. pancreas -- secretes enzymes to digest sugars, fats, and proteins.

 

If the stomach is acidic, why don't we digest ourselves?

1. cells lining stomach not only produce HCl, but also mucous to neutralize acid.

2. cells lining stomach are replaced much more rapidly than other cell types.

3. cells lining stomach have many tight junctions so that acid doesn't leak out.

What about when acidic contents leave the stomach?

4. pancreas secretes basic sol'n to neutralize acid and continue digestion.

 

All respiratory systems share two characteristics:

1. a moist respiratory surface to facilitate gas exchange (gases must be dissolved in liquid in order to enter/exit living cells).

2. a relatively large surface area in contact with the environment (to allow for adequate rates of the diffusion of gases).

 

Pathway of air into the lungs -- From the nose/mouth, air enters pharynx, to larynx (containing vocal cords), to trachea, to bronchi, to bronchioles, to alveoli where gas exchange with capillaries occurs.

A single alveolus and the adjacent capillary are each only 1 cell layer thick, such that is it easy for O2 to diffuse across into the blood stream. The bond between O2 and Hb is weak and reversible, such that when in the tissues, O2 can be released to the cells for respiration. Some O2 is replaced by CO2, but most of the CO2 molecules are converted into bicarbonate ions (-HCO3) for transport to the lungs.

 

Breathing cycle:

1. Inhalation accomplished by making chest cavity larger via diaphragm and intercostal muscle contraction.

2. The enlarged cavity generates negative pressure in lungs which draws air inwards.

3. Exhalation occurs when these muscles relax, compressing the chest cavity, and forcing air out of the lungs.

 

Functions of the circulatory system:

1. gas transport & exchange

2. distribution of nutrients from digestive system to other body areas

3. transport of toxins/wastes to liver (detoxification) & kidneys (excretion)

4. distribution of hormones from endocrine glands to target tissue.

5. regulation of body temperature

6. initial wound healing through clot formation

7. protection from infection via distribution of antibodies & leukocytes.

 

Blood elements -- formed elements from bone marrow

1. erythrocytes (red blood cells), anucleate, transport gases.

2. leukocytes (white blood cells), nucleate, protect body from foreign material

3. platelets (fragments of megakaryocytes), anucleate, cause blood clotting

-- plasma is the fluid bathing the formed elements that contains proteins (immune, clotting, osmotic function), salts, hormones, nutrients, and gases.

 

Flow of blood in mammalian body:

1. blood leaves right ventricle via pulmonary artery to go to lungs where CO2 can be exchanged for O2 in capillary beds of lungs.

2. blood from lungs enters left atrium via pulmonary vein (still w/ O2).

3. blood from left atrium enters left ventricle

4. blood leaves left ventricle via aorta to go to body where it can exchange O2 for CO2 in capillary beds.

5. in body, blood goes from aorta to arteries, to arterioles, to capillaries (gas exchange occurs here), to venules, to veins to the vena cava and back to heart.

6. blood from body (w/ CO2) enters right atrium via vena cava (large vein).

7. blood from right atrium enters right ventricle

 

What keeps the blood flowing in the same direction?

1. valves separating atrial & ventricular chambers prevent backflow when heart contracts.

2. valves in veins that prevent backflow as blood returns to heart.

3. muscle contraction around veins pushes blood through valves towards heart.

 

Evolution of heart structure:

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The Unity of Regulation

The excretory system is effective at several functions:

1. elimination of digestive waste products.

2. regulation of water content in plasma.

3. regulation of ion balance in plasma (esp. Na+, K+, Cl-, Ca+2).

4. regulation of pH of blood.

5. retention of nutrients in blood (esp. sugars and amino acids).

6. secretion of hormones to promote erythrocyte formation.

 

Pathway of urine formation in kidney:

1. blood enters the kidney via renal artery which branches to form a capillary bed called the glomerulus. Water, nutrients, and waste are filtered from the capillary into part of the nephron called the Bowman's capsule.

2. The filtrate then passes along the loop of Henle that is entwined with the capillaries that left the glomerulus. Nutrients and most of the water are reabsorbed into blood. Also, additional wastes are actively transported into the nephron tubule.

3. The wastes are then concentrated in the collecting duct of the nephron which drains to the ureter connecting the kidney to the bladder. This concentration results in a urine solution that is hyperosmotic (4x) to the blood in the surrounding capillaries.

4. The bladder is drained by the urethra that transports the urine out of body.

5. blood containing required concentrations of water, nutrients, and ions returns to the rest of the circulatory system via renal vein.

 

Not all vertebrates use the same structures within kidney which has two functional units: pronephros & opisthonephros (= meso- + meta- nephros).

(a) all embryonic forms use pronephros

(b) nonamniotic adults use segmented opisthonephros

(c) amniotic adults use only metanephric portion of opisthonephros

 

Alternate forms of excretion:

1. mammals, chondrichthyes, few amphibians = urea

2. reptiles, birds = uric acid

3. fish, amphibians = ammonia

 

Other osmoregulatory organs:

1. sweat glands -- secretion of NaCl and H2O for evaporative cooling

2. liver -- regulation of blood glucose levels, toxicant removal, and conversion of ammonia to urea (amniotes only).

 

Excretory systems are closely aligned with reproductive systems — all vertebrates have separate structures producing stuff, but......

(a) nonmammalian vertebrates all have common urogenital opening (cloaca)

(b) nonprimate mammals have separate opening for digestive and urogenital products

(c) female primates w/ separate openings for digestive, excretory, & gonads.

Mechanisms of sex determination:

1. chromosomal sex — a gene on one chromosome initiates gonadal development for particular gender.

2. environmental sex determination — temperature, water potential, or other extrinsic factor influences sex.

 

The pathway of sperm (mammalian).

1. From the seminiferous tubules in the testis where they are formed, sperm travel to the epididymis and vas deferens where they are stored.

2. During sexual arousal, muscles surrounding the epididymis, (vas deferens, and urethra) contract to eject sperm (from the penis).

3. The ejaculated semen is actually the sperm combined with secretions from three other glands along the vas deferens, the seminal vesicle, (the bulbourethral gland, and the prostate gland). These secretions provide nutrients for the sperm (and neutralize the acidity of the female vagina).

 

The pathway of the egg (mammalian).

1. When mature, the egg is released from a follicle w/in an ovary that is suspended in the body cavity.

2. The egg is swept into a fallopian tube where fertilization usually occurs.

(3. If the egg is fertilized, the zygote passes into the uterus where it implants as an embryo into the endometrium that will form the placenta. The suitability of the endometrium as an environment to receive the egg is regulated by hormones from the follicle.)

 

Modes of reproduction:

1. oviparity — egg-laying

2. viviparity —2 types

  : lecithotrophic = retaining a shell-less egg without providing additional nutrients to developing embryo

  : matrotrophic = providing nutrients to embryo via placenta

 

Anatomy of a nerve cell:

1. cell body -- contains most organelles, serves as integrating center.

2. axon -- long, thin portion of cell that transmits AP along length of cell.

3. dendrites -- branched portions of cells that respond to stimuli and conduct AP to cell body.

4. synapse -- space between adjacent neurons across which AP is transmitted via use of chemical neurotransmitter.

Nerve cells function to:

1. receive stimuli from environment (external, internal, other neurons).

2. integrate info. from stimuli and produce appropriate response.

3. conduct action potential (AP, signal) along length of cell.

4. transmit AP to neighboring cell.

 

The nervous system is divided into 2 portions:

1) central nervous system = brain + spinal cord

2) peripheral nervous system = non-CNS neurons enervating muscle, glands and sensory organs; the PNS is subdivided into.......

a) sensory division — detecting stimuli

b) motor division — responding to stimuli; responses fall into 2 categories

i) somatic — voluntary responses (conscious control)

ii) autonomic — involuntary responses that stimulate or suppress activity.

 

Brain structure & function

A. Prosencephalon (forebrain)

1. telencephalon (cerebrum + olfactory bulb) -- sensory & higher mental function (e.g., intellect, communication, memory).

2. diencephalon (thalamus + hypothalamus + pituitary) -- homeostasis, endocrine regulation, emotion.

B. Mesencephalon (midbrain) -- filters & relays sensory info. from all body regions.

C. Rhombencephalon (hindbrain)

1. metencephalon (cerebellum + pons) -- coordination & respiratory rate

2. myelencephalon (medulla oblongata) -- autonomic control of respiration, circulation, swallowing.

 

The Senses

A. Chemoreception (smell & taste)

1. olfactory receptors in nasal passage, sensitive to airborne molecules.

2. taste buds on tongue, sensitive to waterborne molecules.

B. Mechanoreception (movement)

1. specialized nerve endings in skin, sensitive to vibration.

2. free nerve endings in muscles, sensitive to stretching (e.g., in bladder, stomach).

3. hair cells in inner ear, sensitive to vibration or fluid movement; pathway of sound: auditory canal -> eardrum -> hammer -> anvil -> stirrup -> oval window -> cochlea -> hair cells on organ of Corti.

C. Photoreception (wavelengths of light)

1. The pathway of light: cornea - aqueous humor, iris (the opening of which is the pupil), lens (protein, focuses light), vitreous humor, retina which is composed of two types of photoreceptive neurons:

a. rods -- greater #, more sensitive, used for seeing in low light.

b. cones -- lesser #, sensitive to different wavelengths of light, used for seeing color.

2. Infrared wavelengths detected by pit organ in Crotalinae.

D. Thermoreception -- the sense of temperature change, can accommodate.

E. Pain reception -- regardless of the stimulus, our sense of pain is relatively unchanged because the nerve cell type is the same throughout the body.

 

2 types of endocrine action:

1. water-soluble hormones bind to IMP receptor on cell surface to trigger rapid responses via secondary messengers w/in cells (e.g., epinephrine)

2. lipid-soluble hormones pass through cell membrane and nuclear envelope, then binding to protein receptors that activate DNA expression (e.g., steroids like testosterone, estradiol).

 

Important endocrine glands:

1. hypothalamus (base of brain) secretes hormones that regulate endocrine function of pituitary gland.

2. pituitary gland (base of brain) secrete hormones that regulate homeostasis, endocrine function of adrenal glands and gonads, milk production and secretion in mammary glands. E.g., ADH, prolactin, FSH

3. thyroid & parathyroid (neck region) secrete hormones that regulate metabolic and developmental rate, and calcium uptake for bone growth.

4. pancreas (along anterior small intestine) secretes hormones that regulate blood sugar levels. E.g., insulin, glucagon.

4. adrenal glands (on top of kidneys) secrete hormones that regulate metabolic rate. E.g., epinephrine.

5. ovaries secrete hormones that regulate development of 1° and 2° sex characteristics in females. E.g., estradiol, progesterone.

6. testes secrete hormones that regulate development of 1° and 2° sex characteristics in males. E.g., testosterone.

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Functioning in an Aquatic Realm

1. Gills use countercurrent exchange to perform a dual function:

a) respiration – blood flows across gill filaments in direction opposite to water flow such that gas exchange rate is maximized; pump or ram ventilation of buccal chamber; some fish are obligate air-breathers thru gut evaginations.

b) regulating ion balance –actively transport of NH4, Na+, Ca+2 across gill lining depending on whether organism lives in salt or fresh water.

 

2. Locomotion:

a) types of lateral undulation –

(i) anguiliform: flexion of more than half a sinusoidal wavelength

(ii) carangiform: flexion of caudal region of body

(iii) ostraciiform: flexion of tail fin only (body is not flexible)

 

b) overcoming gravity –

(i) positive attack of pectoral fins in water column during forward motion

(ii) swim bladders may serve dual purpose of gas exchange & buoyancy

(iii) static lift enabled by low density lipids

c) overcoming drag in the water –

(i) decrease body length = decreased surface area for viscous drag

(ii) decrease body surface irregularities = decreased viscous drag

(iii) decrease % body length that flexes = decreased inertial drag

(iv) increase aspect ratio = decreased inertial drag (& burst speed)

 

3. Sensory perception:

a) photoreception – spherical lens moved toward/away from retina to focus

b) chemoreception (1° mouth) is relatively sensitive

c) mechanoreception – specialized lateral line system of neuromasts provide info about orientation, water current, motion, pressure.

d) electroreception – modified muscle cells, electrocytes, synchronously generate net positive unidirectional current (_ 600 v) for orientation, communication, or predation.

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END OF MATERIAL FOR 1ST EXAMINATION

 

 

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